I would strongly advise following steps 2 and 3 in the following article BEFORE installing ISA Server 2006 on 32-bit Windows Server 2003 with SP2: http://blogs.technet.com/isablog/archive/2007/03/27/isa-server-and-windows-server-2003-service-pack-2.aspx. BTW, step 2 only needs to be done on multi-core multi-processor servers (mine were 2 socket dual core HP DL380's).
I had all sorts of issues before implementing these fixes - I couldn't tell you wish one solved the problems but it is probably a good idea to do both just in case.
If implementing step 2 and setting the mask to assign NICs to separate processors, I assigned NIC1 port A to processor thread 0, NIC1 port B to processor thread 1, NIC2 port A to processor thread 2 and NIC2 port B to processor thread 3.
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